FIGURE 5.

Akt phosphorylation is stimulated by NRG4 in vitro and in vivo. A, YAMC-B4 cells were stimulated with NRG4 for 10 min, and whole cell lysates were prepared. B, cells were treated with 0–1000 ng/ml NRG4. C, mice were injected with NRG4 with or without TNF + IFN-γ; after 24 h, epithelial homogenates were prepared. Expression and phosphorylation of the indicated molecules were determined by Western blot analysis. D, Akt phosphorylation in fixed, paraffin-embedded colonic tissue was assessed by immunofluorescence analysis. Data are representative of at least three independent experiments or mice per condition. P-Akt, phospho-Akt.