FIGURE 7.

NRG4 levels are decreased in inflammatory bowel disease. A and B, qPCR analysis for NRG4 (A) and HRG-1β (B) gene expression was performed on TissueScan Crohn's/colitis qPCR arrays. Relative mRNA levels were calculated using the 2^−ΔΔCT method with β-actin as the reference. C, colonic homogenates from wild type controls (WT) or IL-10^−/− mice were subjected to Western blot analysis for ErbB4, phospho-ErbB4 (P-ErbB4), and NRG4. Results shown are representative of four mice per genotype. UC, ulcerative colitis.