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. 2012 Aug 16;18(23-24):2590–2600. doi: 10.1089/ten.tea.2011.0588

FIG. 8.

FIG. 8.

Pressure-dependent endothelial sprout formation was sensitive to extracellular levels of FGF-2. Representative images of BAEC on Cytodex beads in collagen gels exposed to either 20- (B) or 40-mmHg (D) static pressures in the presence of 2.5 ng/mL FGF-2 for 3 days. Images of time-matched controls are provided for each pressure level (A and C). The total number of sprouts (E) as well as the number of sprouts extending 75 μm (F) from endothelial monolayers under pressure stimulation were manually counted per bead for 15 beads and normalized to those of controls. Vertical bars in (E) and (F) are mean fold changes in sprout formation±standard error; n=4 to 5. #p<0.05 compared to levels of matched controls assigned a value of 1 using one-sample t-test.