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A small subset of cultured neurons were transfected with a viral construct containing synaptophysin-GFP (Sph-GFP), to visualize presynaptic terminals, and tetanus toxin light chain (TeNT), to greatly diminish neurotransmitter release. Individual postsynaptic neurons were transfected with a red fluorophore (mCherry), allowing the silenced synapses to be visually identified by overlap of red and green signals.
