FIG. 5.

Antiluciferase staining of sections from a particle-infused femur and a saline-infused femur at day 28 postcell injection. All sections were obtained from the diaphyseal cortex. DAPI identified all cells, antiluciferase antibody identified exogenous cells, and an overlay identified any costaining. Sections were imaged at 20×, and cells that were positive for both DAPI and antiluciferase staining were counted using ImageJ Software. A greater number of exogenous cells was found in sections from particle-infused femora. The increase in luciferase-positive staining cells in the PE-infused femur confirms that MC3T3 cells were recruited systemically to the site of wear-particle-induced osteolysis.