FIG. 6.

Immunohistochemical staining of a particle-infused femur (left block) and a saline-infused femur (right block). All sections were obtained from the diaphyseal cortex. DAPI identified all cells (A), antihuman vitronectin receptor αVβ3 identified osteoclasts (B), antiosteocalcin identified osteoblasts (C), and overlaying all three identified any costaining (D). Osteoclasts reside along the trabecular bone and the endosteal cortices (representative areas denoted by *), predominantly so in the case of saline-infused femora. The experimental femora had a much greater extent of osteoblast staining (representative areas indicated with #). All images are 200×magnification. Color images available online at www.liebertpub.com/tea