FIG. 1.

Decellularized macaque lungs are efficiently generated using the Triton/SDC method. (A) Cadaveric lungs (pictured collapsed, ex vivo) from rhesus macaques undergoing euthanasia were decellularized by the Triton X-100/SDC method. (B) For decellularization, the cadaveric lungs were subjected to sequential instillation of detergents, hypertonic saline, and DNase into the airway via the trachea and through the vasculature via the right ventricle and pulmonary artery over a period of 3 days. (C) The resulting decellularized lungs retain the ultrastructural and microanatomical features of native lungs including maintenance of large air-conducting passages, blood vessels, and lung parenchyma. SDC, sodium deoxycholate. Color images available online at www.liebertpub.com/tea