Table 1.
Proteomics Database Search Results Sorted for Frequency of Peptides (Greatest to Least) and Rank of Extracellular Matrix Proteins Identified
|
Native macaque lung |
Decellularized macaque lung |
||||
|---|---|---|---|---|---|
| Rank | No. of peptides | Description | Rank | No. of peptides | Description |
| 1 | 80 | Neuroblast differentiation-associated protein AHNAK | 1 | 18 | Isoform 1 of Annexin A2 |
| 2 | 52 | Filamin A, alpha Ing=2607 | 2 | 18 | Isoform 6 of myoferlin |
| 3 | 52 | Talin-1 | 3 | 14 | Fibrillin-1 |
| 4 | 51 | Isoform 2 of spectrin alpha chain, brain | 4 | 11 | Collagen alpha-3(VI) chain isoform 4 precursor |
| 5 | 50 | Isoform 1 of myosin-9 | 5 | 8 | Annexin A6 |
| 6 | 34 | Isoform long of spectrin beta chain, brain 1 | 6 | 8 | cDNA FLJ90527 fis, clone NT2RP4004167, highly similar to 5′-nucleotidase |
| 7 | 30 | Isoform 2 of Clathrin heavy chain 1 | 7 | 8 | Isoform 1 of myosin-10 |
| 8 | 30 | Myosin-11 isoform SM2A | 8 | 8 | Isoform beta-1B of integrin beta-1 |
| 9 | 27 | Isoform 1 of vinculin | 9 | 8 | Keratin, type II cytoskeletal 1 |
| 10 | 26 | Cytoplasmic dynein 1 heavy chain 1 | 10 | 8 | Laminin subunit gamma-1 |
| 141 | 7 | Lumican | 15 | 7 | Basement membrane-specific heparin sulfate proteoglycan crore protein variant Ing=4374 |
| 200 | 5 | Collagen alpha-3(VI) chain isoform 4 precursor | 17 | 7 | Laminin subunit alpha-3 isoform 3 |
| 264 | 4 | Collagen alpha-1(VI) chain | 24 | 5 | Chondroadherin |
| 382 | 3 | Isoform 2 of Collagen alpha-1(XIV) chain | 26 | 4 | Collagen alpha-2(IV) chain |
| 410 | 3 | Prolargin | 31 | 4 | Iosform A of Decorin |
| 486 | 2 | cDNA FLJ35535 fis, clone SPLEN2002419, highly similar to EGF-containing fibulin-like extracellular matrix protein 1 | 33 | 4 | Laminin subunit alpha-5 |
| 527 | 2 | Fibulin-5 | 34 | 4 | Laminin subunit beta-2 |
| 573 | 2 | Isoform A of fibulin-1 | 43 | 3 | cDNA FLJ60269, highly similar toHomosapiensnephronectin (NPNT), mRNA |
| 769 | 1 | Collagen alpha-2(IV) chain | 45 | 3 | Collagen alpha-1(VI) chain |
| 861 | 1 | Isoform 2C2A′ of collagen alpha-2(VI) chain | 46 | 3 | Collagen alpha-2(I) chain |
| 48 | 3 | Fibulin-5 | |||
| 62 | 2 | cDNA FLJ51266, highly similar to vitronectin | |||
| 65 | 2 | Isoform 1 of collagen alpha-6(VI) chain | |||
| 94 | 1 | cDNA FLJ53292, highly similar toHomo sapiensfibronectin 1 (FN1), transcript variant 5, mRNA Ing=1014 | |||
| 99 | 1 | cDNA FLJ61309, highly similar to Laminin beta-3 chain | |||
| 101 | 1 | Collagen alpha-4(IV) chain | |||
| 116 | 1 | Isoform 1 of collagen alpha-1(II) chain | |||
| 122 | 1 | Isoform 2 of collagen alpha-3(IV) chain | |||
| 127 | 1 | Isoform 3 of aggrecan core protein | |||
| 131 | 1 | Isoform long of laminin subunit gamma-2 | |||
Entries in bold represent peptides identified as being derived from the extracellular matrix.