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Regulation of matrix metalloproteinase (MMP1 and MMP2) activity by culture condition. hVFF grown on TCP, 2D HA, and 3D HA were starved for 24 h before the addition of various doses of TGF-β1 for 48 h. Equal amounts of cell supernatant were analyzed by the Zymogram assay. (A) MMP1 activity assay was performed by casein zymogram. (B) MMP2 activities were assayed by gelatin zymogram.
