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Expression of TGF-β receptors in hVFF were detected by immunocytochemistry staining. TGFBRI (A, red) and TGFBRII (B, green) were observed in the cells on TCP (a, d), 2D HA (b, e), and in 3D HA (c, f) conditions treated with either medium alone (a, b, c) or 5 ng/mL TGF-β1 (d, e, f) for 48 h. Nuclei were counter-stained with DAPI. Scale bar: 60 μm.
