Table III.
Species | Endogenous DMSP | 35S Uptake | [35S]DMSP-amine | [35S]DMSP |
---|---|---|---|---|
μmol g−1 fresh weight | kBq | Bq | ||
S. alterniflora | 29.0 | 18.3 | 389 | 8180 |
S. patens | <0.03 | 28.6 | <30 | <30 |
T. aestivum | <0.03 | 37.0 | <30 | <30 |
Z. mays | <0.03 | 36.6 | <30 | 90 |
B. glaucescens | <0.03 | 36.3 | <30 | <30 |
O. compositus | <0.03 | 37.6 | <30 | <30 |
C. dactylon | <0.03 | 35.1 | <30 | <30 |
P. purpureum | <0.03 | 36.8 | <30 | 70 |
C. selloana | <0.03 | 21.6 | <30 | <30 |
Leaf tissue (0.2 g fresh weight) of eight grasses shown not to accumulate DMSP was incubated for 6 h with 38.9 kBq (0.12 nmol) of [35S]SMM; S. alterniflora was included as a benchmark. Uptake was calculated from 35S disappearance from the medium. The radioactivity values shown are per 0.2 g fresh weight and have been corrected for recovery. Endogenous DMSP contents were determined on duplicate 30-mg fresh weight leaf samples. The comparison between S. alterniflora and S. patens was repeated, with similar results.