Figure 1.

Time course of expression of haematopoietic regulator genes during ES cell differentiation. (A) Schematic outline of the haematopoietic differentiation model. Haemangioblast (Flk1+, Bry+) cells are sorted from embryoid bodies on day 0 and during a 4-day blast culture through the stage of the haemogenic endothelium give rise to haematopoietic progenitors, which can be differentiated into macrophages (CD11b+, F4/80+). Relevant surface markers of sorted cell populations are indicated. (B) Relative expression analysis of haematopoietic regulator genes in purified cells from all stages of differentiation. Flk1− cells served as control and experiments were carried out at least in triplicates where STDEV was applied, otherwise the average of two biological duplicates and the respective values are shown.