Figure 5.

FLI1 and SCL/TAL1 binding profiles in haematopoietic c-kit+ precursor cells. (A) Venn diagram showing the intersection of ChIP-sequencing data of FLI1 and SCL/TAL1 in c-kit+/CD41+/Tie2− (c-kit+) wild-type progenitor cells prepared on day 4 of blast differentiation. (B) UCSC genome browser screenshots providing examples of genes where the binding pattern of FLI1 and SCL/TAL1 changes during differentiation from the uninduced haemogenic endothelium (HE) to c-kit+ progenitors. (C) Venn diagrams showing the overlap of peaks of SCL/TAL1 (left) and FLI1 (right) between the uninduced HE and wild-type c-kit+ progenitors. (D) Hierarchical clustering of transcription factor binding sequences in different cell types (data sets from this study are highlighted in blue). Based on similar binding patterns of the different ChIP-seq data, a correlation matrix was generated and sequence similarities are displayed after hierarchical clustering and using a colour code.