Figure 6.

Identification of the domains regulating NICD protein stability. (A) Expression pattern of the YFP::LacZ as control, (B) the complete LIN-12 intracellular domain (NICD::GFP), (C) a C-terminal truncation of 87 amino acids containing the PEST domain (NICD::GFPΔCT), (D) an N-terminal deletion of 384 amino acids removing the RAM domain and ANK repeats (NICD::GFPΔNT), and (E) an internal deletion of 324 amino acids removing the ANK repeats (NICD::GFPΔANK). All constructs were expressed using the VPC-specific bar-1 promoter fragment (Natarajan et al, 2004) and inserted via MosSCI as single copy transgenes at the same location on LGII. Expression was scored in the descendants of P5.p, P6.p, and P7.p at the Pn.px stage. For each construct, a quantification of the GFP levels is shown in the bar graphs in the center. Error bars indicate the standard error as described in Materials and methods. Representative animals for each construct are shown in the right panels. The brackets indicate the position of the P6.p descendants. The scale bar represents 10 μm. Source data is available for this figure in the Supplementary Information.

Source data for Figure 6 ^{(46.5KB, xls)}