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. 1998 May;117(1):283–292. doi: 10.1104/pp.117.1.283

Figure 6.

Figure 6

The NR (A), PEPCase (B), or SPS (C) transcript levels in leaves of water-replete controls (WR) or water-deprived plants (WD) at 3 d (1), 5 d (2), and 7 d (3) after withholding irrigation or 24 h after irrigation to soil capacity of maize plants that had been deprived of water for 7 d (RW). Denatured total RNA (10 μg) samples were fractionated on a formaldehyde-agarose gel (1.5% agarose) and transferred onto a membrane. The RNA blots were probed with the partial 2.2-kb cDNA clone pZmnrl encoding NR of maize (Gowrie and Campbell, 1989), a partial 1.0-kb cDNA clone corresponding to PEPCase of maize (Sheen and Bogorad, 1987), or a full-length 3.4-kb cDNA clone encoding SPS of maize (Worrell et al., 1991).