Skip to main content
. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2012 Mar 8;32(5):1167–1177. doi: 10.1161/ATVBAHA.112.247668

Figure 5. Elevation of VEGF-A by alcohol activates caspase-1.

Figure 5

A, Changes in the expression of VEGFR-2 (green), caspase-1 (red), DAPI (blue) in hBECs after exposure to EtOH (50 mM), Ki8751 (10 μM) and VEGF-A (100 ng/mL) for 24 hr. Scale bar =20 μm. B-C, Immunoblot analysis to show the alterations in caspase-1 protein levels in: (B) hBECs culture following treatment with respective test compounds, and (C) brain cortical tissue and microvessel homogenates protein. Bar graphs show the results, which are expressed as ratio of caspase-1 to that of β-actin bands. Values are mean ±SEM; n=4 to 5. **p<0.01; ***p<0.001 vs untreated or control in B and C; #p<0.05; ##p<0.01 vs EtOH (second bar) in B.