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. 2012 Nov 19;209(12):2165–2181. doi: 10.1084/jem.20121090

Figure 3.

Figure 3.

Loss of S1pr1 increases the size but has no effect on the positioning and motility of MKs in vivo. (A) Representative MP-IVM images of YFP+ or EGFP+ MKs (green) in BM. BM microvasculature was visualized by intravenous injection of TRITC-dextran (red). Left, naive (nontransplanted) S1pr1+/+CD41-YFPki/+; middle left, S1pr1+/+;Pf4-EYFP; middle right, S1pr1+/+;lenti-GPIbα-EGFP BM chimaeras; right, S1pr1+/+;CD41-YFPki/+ FL chimaeras. Bars, 20 µm. (B–D) Volumes (B), distances from sinusoids (C), and the instantaneous lateral (x-y) velocity (D) of MKs in the indicated groups. Red lines in C indicate medians; red lines in D indicate means. Error bars represent SEM. n = 13–46 MKs per genotype. Data are pooled from three mice each group. P-values among the different groups in B–D are >0.05. (E) Surface area of MKs in BM. (F) Distance of MKs from BM sinusoids. (E and F) Red lines indicate medians. (G) Instantaneous lateral (x-y) velocity of MKs. Red lines indicate means. (E–G) Data are pooled from three mice each group.