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. 2012 Nov 19;209(12):2263–2276. doi: 10.1084/jem.20121505

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Figure 8. — Analysis of peripheral CD8 T cells from IL-7Rα–cKO and γ_c-cKO mice. (A) Absolute cell number of splenic CD8⁺ T cells. CD8⁺ T cell numbers were determined from splenocytes for Cre⁻ control (black bar), IL-7Rα–cKO (blue bar), and IL-7Rα–cKO–IL-15^−/− (green bar) mice. CD8⁺ T cells from IL-7Rα–cKO and IL-7Rα–cKO–IL-15^−/− mice were specifically gated on IL-7Rα–deleted cells. Data represent the mean of at least seven individual mice from at least four independent experiments, and error bars represent SEM. ****, P < 0.0001. (B) TCR-β⁺ CD8⁺ splenocytes were analyzed for frequency of naive and CD44^hiCD122^hi memory phenotype cells among IL-7Rα–deleted cells (blue) compared with Cre⁻ controls (black). Data are representative of three independent experiments. (C) Absolute CD8⁺ T cell numbers were determined from splenocytes from Cre⁻ control (black bar), and γ_c-cKO (red bars) mice. CD8⁺ T cells from γ_c-cKO mice were specifically gated on γ_c-undeleted (labeled γ_c⁺) or γ_c-deleted (labeled γ_c^Δ) cells. Data represent the mean of at least seven individual mice from at least three independent experiments, and error bars indicate SEM. ***, P < 0.001; ****, P < 0.0001. (D) TCR-β⁺ CD8⁺ splenocytes were analyzed for frequency of naive and CD44^hiCD122^hi memory phenotype cells among γ_c⁺ (escapees) cells from γ_c-cKO mice (red) compared with Cre⁻ controls (black). Data are representative of three independent experiments.