Figure 4.

Minocycline effect on vertical climbing in male Drosophila melanogaster. Climbing index = % of flies moved to the top vial. Twenty flies were placed at the bottom of a clean 4-inch glass vial over which a second identical vial was inverted. After 30 s the two vials were separated. The climbing index was expressed as the percentage of the flies that climbed to the top vial relative to the total number of flies tested. Four climbing trials (akin to sampling with replacement) were conducted per vial. There were 35 climbing trials for Control group (total number of tested flies = 700); and 25 trials for minocycline group (total number of tested flies= 500). Mean ± SEM; p < 0.01: minocycline VS control group, two-way ANOVA, Bonferroni adjusted.