Figure 1.

Circulating human neutrophils and monocytes express αβT cell receptor-like recombinatorial immune receptors (TCRLαβ). The confocal fluorescence immunocytochemistry images show ∼5–8% subpopulations of circulating neutrophils (left) and monocytes (right) that express the TCRLαβ (arrows). TCRLαβ positive neutrophils and monocytes display yellow and red fluorescence, respectively. Nuclei are counterstained in the left image (blue). CD15⁺ and CD14⁺ purified peripheral blood neutrophils and monocytes, respectively, were isolated from representative healthy donors and immunostained using antibodies against the TCRαβ (yellow, neutrophils; red; monocytes). Monoytes were costained for MHC-II (green). Adapted from Puellmann et al. [9] and Beham et al. [13].