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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1980 Oct;77(10):5716–5719. doi: 10.1073/pnas.77.10.5716

NH2-terminal amino acid sequence of human fibroblast interferon.

S Stein, C Kenny, H J Friesen, J Shively, U Del Valle, S Pestka
PMCID: PMC350140  PMID: 6160579

Abstract

The purification of human fibroblast interferon by chromatography on Blue Sepharose and high-performance liquid chromatography is described. The amino acid composition and a partial sequence of the homogeneous protein are reported. The NH2 terminus was determined to be NH2-Met1-Ser-Tyr-Asn-Leu-Leu-Gly-Phe-Leu-Gln-Arg-Ser-Ser-Asn-Phe-Gln-X-Gln-Lys.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Berthold W., Tan C., Tan Y. H. Purification and in vitro labeling of interferon from a human fibroblastoid cell line. J Biol Chem. 1978 Jul 25;253(14):5206–5212. [PubMed] [Google Scholar]
  2. Böhlen P., Stein S., Stone J., Udenfriend S. Automatic Monitoring of primary amines in preparative column effluents with fluorescamine. Anal Biochem. 1975 Aug;67(2):438–445. doi: 10.1016/0003-2697(75)90316-4. [DOI] [PubMed] [Google Scholar]
  3. Cavalieri R. L., Havell E. A., Vilcek J., Pestka S. Synthesis of human interferon by Xenopus laevis oocytes: two structural genes for interferons in human cells. Proc Natl Acad Sci U S A. 1977 Aug;74(8):3287–3291. doi: 10.1073/pnas.74.8.3287. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Havell E. A., Vilcek J. Production of high-titered interferon in cultures of human diploid cells. Antimicrob Agents Chemother. 1972 Dec;2(6):476–484. doi: 10.1128/aac.2.6.476. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hunkapiller M. W., Hood L. E. Direct microsequence analysis of polypeptides using an improved sequenator, a nonprotein carrier (polybrene), and high pressure liquid chromatography. Biochemistry. 1978 May 30;17(11):2124–2133. doi: 10.1021/bi00604a016. [DOI] [PubMed] [Google Scholar]
  6. Jankowski W. J., von Muenchhausen W., Sulkowski E., Carter W. A. Binding of human interferons to immobolized Cibacron Blue F3GA: The nature of molecular interaction. Biochemistry. 1976 Nov 16;15(23):5182–5187. doi: 10.1021/bi00668a036. [DOI] [PubMed] [Google Scholar]
  7. Knight E., Jr, Hunkapiller M. W., Korant B. D., Hardy R. W., Hood L. E. Human fibroblast interferon: amino acid analysis and amino terminal amino acid sequence. Science. 1980 Feb 1;207(4430):525–526. doi: 10.1126/science.7352259. [DOI] [PubMed] [Google Scholar]
  8. Levy W. P., Shively J., Rubinstein M., Del Valle U., Pestka S. Amino-terminal amino acid sequence of human leukocyte interferon. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5102–5104. doi: 10.1073/pnas.77.9.5102. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Rubinstein M., Rubinstein S., Familletti P. C., Miller R. S., Waldman A. A., Pestka S. Human leukocyte interferon: production, purification to homogeneity, and initial characterization. Proc Natl Acad Sci U S A. 1979 Feb;76(2):640–644. doi: 10.1073/pnas.76.2.640. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Stein S., Böhlen P., Stone J., Dairman W., Udenfriend S. Amino acid analysis with fluorescamine at the picomole level. Arch Biochem Biophys. 1973 Mar;155(1):202–212. doi: 10.1016/s0003-9861(73)80022-0. [DOI] [PubMed] [Google Scholar]
  11. Taira H., Broeze R. J., Jayaram B. M., Lengyel P., Hunkapiller M. W., Hood L. E. Mouse interferons: amino terminal amino acid sequences of various species. Science. 1980 Feb 1;207(4430):528–530. doi: 10.1126/science.7352261. [DOI] [PubMed] [Google Scholar]
  12. Tan Y. H., Barakat F., Berthold W., Smith-Johannsen H., Tan C. The isolation and amino acid/sugar composition of human fibroblastoid interferon. J Biol Chem. 1979 Aug 25;254(16):8067–8073. [PubMed] [Google Scholar]
  13. Tucker P., Pestka S. De novo synthesis and glycosylation of the MOPC-46B mouse immunoglobulin light chain in cell-free extracts. J Biol Chem. 1977 Jul 10;252(13):4474–4486. [PubMed] [Google Scholar]
  14. Wittmann-Liebold B. Amino acid sequence studies on ten ribosomal proteins of Escherichia coli with an improved sequenator equipped with an automatic conversion device. Hoppe Seylers Z Physiol Chem. 1973 Oct-Nov;354(10-11):1415–1431. doi: 10.1515/bchm2.1973.354.2.1415. [DOI] [PubMed] [Google Scholar]
  15. Wittmann-Liebold B., Graffunder H., Kohls H. A device coupled to a modified sequenator for the automated conversion of anilinothiazolinones into PTH amino acids. Anal Biochem. 1976 Oct;75(2):621–633. doi: 10.1016/0003-2697(76)90117-2. [DOI] [PubMed] [Google Scholar]
  16. Zoon K. C., Smith M. E., Bridgen P. J., Anfinsen C. B., Hunkapiller M. W., Hood L. E. Amino terminal sequence of the major component of human lymphoblastoid interferon. Science. 1980 Feb 1;207(4430):527–528. doi: 10.1126/science.7352260. [DOI] [PubMed] [Google Scholar]

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