Figure 1.

Transfection was successful and levels of JMJD2A mRNA and protein were both down-regulated. (A) Green fluorescent cells transfected with FAM-siRNA under fluorescence microscope. (B) Column diagram analysis for mRNA levels of JMJD2A. The JMJD2A-specific siRNA group exhibited a reduction in JMJD2A mRNA levels in MCF-7 cells. (C) Western blot analysis for JMJD2A protein. (D) Column diagram analysis for optical density by western blotting. JMJD2A protein levels were downregulated in the siRNA group. ^*P<0.05 compared with blank and negative control groups.