Figure 4.

PCa-MSCs were induced to differentiate into bone cells (alkaline phosphatase). (a) The cell morphology of PCa-MSCs changed from a spindle-shaped to a flat-shape at 10 days as detected under microscopy (magnification, ×40). (b) More red alkaline grain-containing acid enzyme-positive granules in the cytoplasm at 10 days were noted under microscopy (magnification, ×100). (c) In the normal controls, alkaline phosphatase expression was almost negative as noted under microscopy (magnification, ×100).