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Gel filtration HPLC analysis of the crystals of m5U monitored at 280 nm. Typical chromatograms of (a) crystalline m5U obtained in the presence of denatured DAFP-1, (b) reed-like amorphous m5U precipitates obtained in the presence of DAFP-1, (c) normal orthorhombic crystalline m5U modified using DAFP-1 in the presence of m5U seed crystals. The retention times for m5U and DAFP-1 are 13.1 min and 11.5 min, respectively. Insets: enlarged portions (denoted by asterisks).
