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. 2012 Nov 19;7(11):e49862. doi: 10.1371/journal.pone.0049862

Figure 5. Changes in muscle ultrastructure after Orai1 knockdown.

Figure 5

Longitudinal section of FDB muscles under transmission EM of the (A) wt muscle transfected with control vector. Scale bar is 200 nm. Black arrows designate triads at A-I junctions and white arrows designate triad-defect A-I junctions; (B) mdx muscle transfected with control vector. Red arrows designate the dense triads at A-I junctions; (C) abnormal features observed in muscles with Orai1 knockdown. Left: disoriented triad junctional structure (white arrows); right: thick and rough reticulation as compared to the fine SR network structure in wt (A) and mdx control (B) muscle; (D) Statistical results summarize the percentage of triad-containing A-I junctions in muscle bundles from wt control, wt Orai1 knockdown, mdx control and mdx Orai1 knockdown. Data are mean ± S.E.M. *P<0.05 compared to wt control and # P<0.05 compared to mdx control.