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. 2012 Nov 19;7(11):e49581. doi: 10.1371/journal.pone.0049581

Figure 4. GLI1 induces EMT in HCC ells.

Figure 4

(A) As assessed by qRT-PCR, overexpression of GLI1 decreases E-cadherin mRNA expression and increases the mRNA expression of both N-cadherin and Vimentin in Huh7 cells, while knockdown of GLI1 increases E-cadherin mRNA expression and decreases the mRNA expression of both N-cadherin and Vimentin in SNU398 cells. (B) As assessed by Western immunoblotting followed by densitometry analysis, overexpression of GLI1 decreases E-cadherin expression and increases the expression of both N-cadherin and Vimentin in Huh7 cells, while knockdown of GLI1 increases E-cadherin expression and decreases the expression of both N-cadherin and Vimentin in SNU398 cells.