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. 2012 Nov 19;7(11):e50018. doi: 10.1371/journal.pone.0050018

Figure 2. Representative ChR2 photocurrent traces recorded at −120 mV.

Figure 2

Black bars indicate illumination with 473 nm light. (A) Normalized photocurrent traces. Mutant photocurrents were normalized to WT ChR2 on the same day in 115 mM Na+ solution at pH 9. Values are reported as mean±S.E.M (n = 3–9). Statistically significant values are denoted by a *(*P<0.001; **P<0.04). (B) Reduced photocurrent for I197S compared to WT ChR2 on the same day. M255S and P234S had similar results. (C) Western blot of I197S, P234S, and M255S ChR2 mutants. ChR2-HA tagged band appears at ∼35 kDa. (D) Typical electrophysiological recordings for single serine mutants. Mutants retained inward rectification of cations, but with altered permeability, kinetics, and/or inactivation ratios.