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Changes in reversal potential were calculated by subtracting Erev in cation X+ from Erev in Na+ (mean±S.E.M; n = 7–25). Permeability ratios were calculated using changes in reversal potential. Values are reported as mean±S.E.M (n = 7–25). For significance testing, mutant ChR2 values were compared to WT in the same solution. Statistically significant values are denoted by a *(P<0.001).
