Figure 5. Steady-state to peak current ratios for WT and G181S ChR2.

Ratios were calculated after normalization to −1 µA at −120 mV in cationic solutions (115 mM XCl, 2 mM BaCl₂, 1 mM MgCl₂, Tris; pH 9; n = 14–18). Significant differences are marked with a *(P<0.05). (A) Comparison of WT and G181S photocurrents in Na⁺ solution. (B) I_ss/I_p comparison for WT and G181S in different cationic solutions. Wild-type ChR2 is colored white while G181S is colored black.