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. 2012 Nov 19;7(11):e49577. doi: 10.1371/journal.pone.0049577

Figure 2. Overexpressed ESE-3a and ESE-3b are located in the nucleus of HeLa cells.

Figure 2

HeLa cells were transfected with expression vectors coding for ESE-3a-EGFP and ESE-3b-EGFP. After 24 h, cells were fixed and subjected to immunocytochemistry using a monoclonal antibody specific for human ESE-3. The ESE-3a- and ESE-3b-constructs localized exclusively to the nucleus as revealed by both the intrinsic green fluorescence and the ESE-3 immunoreactivity. One representative experiment out of three is shown. Scale bar: 50 µM.