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. 2012 Nov 19;7(11):e47520. doi: 10.1371/journal.pone.0047520

Figure 5. Intracellular staining for IFN-γ by flow cytometry.

Figure 5

MiaPaCa-2 (or Mia) and L3.6pl cells were either pretreated with AT-101 for 24 h or left untreated and a subsequent 4 h incubation with ATC or aATC in the presence of GolgiStop followed by staining for intracellular IFN-γ by flow cytometry. Y-axis shows the counts and the X-axis of each histogram shows the percentage of fluorochrome positive cells. B). Culture supernatants from AT-101 sensitized PC cells co-cultured with ATC or aATC in the absence of GolgiStop were used to detect the secreted IFN-γ in the culture supernatants, and data are presented as mean ± SD from one representative experiment. Y-axis shows the counts and the X-axis of each histogram shows the percentage of fluorochrome positive cells.