Figure 4. Complex 1 suppresses NAE-regulated NF-κB-dependent luciferase reporter gene expression in a dose-dependent manner.

Caco-2 cells were transfected with the NF-κB-dependent luciferase reporter p3EnhConA-Luc gene, treated with 1 for 16 hours, and then stimulated with 5 ng/ml of TNF-α for 3 hours. Luciferase expression was measured and normalized with β-galactosidase activity. Results are expressed as fold change compared to TNF-α stimulation alone and the errors bar show the standard derivation of triplicate results. MLN4924 was included for comparison.