Figure 3. SUZ12 knockdown inhibits the growth of human EOC cells in vivo.
A, SKOV3 cells were transduced with luciferase-encoding hygromycin-resistant retrovirus together with a puromycin-resistant shSUZ12 encoding lentivirus or control. 3×105 drug-selected cells were unilaterally injected into the periovarian bursa sac of the female immunocompromised mice (n = 9 for each of the groups.). The radiance of luciferase bioluminescence, an indicator of the rate of tumor growth, was measured every other week from week 2 until week 8 by using the IVIS imaging system. Shown are images taken at week 2 and week 8, respectively. B, quantification of tumor growth from the indicated cells. * p=0.009 compared with controls. C, 1×106 drug-selected control and indicated shSUZ12-expressing SKOV3 cells were inject subcutaneously (s.c.) into immunocompromised mice (n = 5 for each of the groups). 5 weeks postinjection, tumors were surgically removed from mice. D, quantitation of C, the size of tumors was measured. Mean of tumor sizes with SEM. P<0.001 compared with controls. E, xenografted tumors formed by control or indicated shSUZ12-expressing SKOV3 cells were sectioned and stained for SUZ12 expression. Bar = 100 μm.