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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Mol Cancer Res. 2012 Sep 10;10(11):1462–1472. doi: 10.1158/1541-7786.MCR-12-0335

Figure 5. shSUZ12 resistant HA-tagged SUZ12 rescues apoptosis induced by SUZ12 knockdown.

Figure 5

A, SKOV3 cells were infected with a lentivirus encoding shSUZ12 (#1) together with control or an shSUZ12 resistant SUZ12 that expresses wild type SUZ12 protein. Expression of SUZ12 mRNA was determined by RT-PCR using primers designed to its 3′ UTR (only amplifies endogenous but not ectopic SUZ12 mRNA) or its open reading frame (ORF, amplifies both endogenous and ectopic SUZ12 mRNA). B, same as A, but examined for expression of HA, SUZ12, H3K27Me3, GAPDH and markers of apoptosis including cleaved lamin A, caspase 3 and PARP p85.