Skip to main content
. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Mol Cancer Res. 2012 Sep 10;10(11):1462–1472. doi: 10.1158/1541-7786.MCR-12-0335

Figure 6. HRK is a novel SUZ12 target gene whose upregulation contributes to apoptosis induced by SUZ12 knockdown.

Figure 6

A, HRK mRNA expression was determined in SKOV3 cells expressing indicated shSUZ12 or control by qRT-PCR. Expression of the housekeeping gene β-2-microglobulin was used to normalize mRNA expression. * p<0.001 compared with controls. B, SKOV3 cells expressing shSUZ12 (#1) or control were subjected to ChIP analysis using antibodies against H3K27Me3 or SUZ12 using primers targeted to HRK genomic locus. A isotype matched IgG was used as a negative control and an anti-core histone H3 antibody was used as a positive control for ChIP analysis. C, SKOV3 cells express shSUZ12 (#1) with or without transfection with siRNA to the human HRK gene (siHRK). Expression of HRK mRNA in the indicated cells was determined by qRT-PCR. Expression of the housekeeping gene β-2-microglobulin was used to normalize mRNA expression. D, same as A, but examined for expression of SUZ12, H3K27Me3, H3K9Me3, GAPDH and markers of apoptosis including cleaved lamin A, caspase 3 and PARP p85.