Table 1.

NMR chemical shift assignments for 20,24(OH)₂D3. (Solvent: CD₃OD)

Atom	20,24(OH)2D3		20(OH)D3
	1H	13C	1H	13C
1	2.11α, 2.41β	33.5	2.11α, 2.40β	32.2
2	1.96α, 1.53β	36.5	1.97α, 1.53β	35.2
3	3.76	70.4	3.76	69.2
4	2.52α, 2.18β	46.8	2.53α, 2.19β	45.6
5	NA	136.4	NA	136.3
6	6.22	122.5	6.21	121.2
7	6.03	119.3	6.02	118.0
8	NA	141.1	NA	141.0
9	1.68α, 2.84β	28.4	1.68α, 2.85β	28.4
10	NA	146.2	NA	145.6
11	1.55α, 1.69β	24.2	1.56α, 1.66β	23.1
12	1.36α, 2.10β	42.3	1.37α, 2.07β	40.9
13	NA	45.4	NA	45.6
14	2.01	57.6	2.00	56.4
15	1.77	23.1	1.51	21.7
16	1.68α, 1.77β	23.0	1.69α, 1.78β	21.7
17	1.69	60.4	1.67	58.5
18	0.70	14.1	0.69	12.8
19	4.75, 5.04	112.4	4.74, 5.04	111.3
20	NA	74.7	NA	74.5
21	1.24	25.9	1.23	24.8
22	1.36	39.6	1.32, 1.46	43.9
23	1.74, 1.38	41.0	1.33	21.7
24	3.22	78.2	1.16	39.6
25	1.62	33.5	1.55	27.8
26	0.93	19.3	0.89	21.7
27	0.92	18.5	0.89	21.7

NA – Not applicable (ternary carbons).