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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: Nat Immunol. 2012 Oct 14;13(12):1196–1204. doi: 10.1038/ni.2432

Figure 5.

Figure 5

The Ebf1 locus is closely associated with the nuclear lamina in pre-pro-B cells. (a) Immuno-FISH in nuclei derived from pre-pro-B and pro-B cells using a fluorescently labeled BAC encoding the Ebf1 domain as in previous figure and lamin antibodies. Digitally magnified pictures of nuclear location of the Ebf1 locus in pre-pro-B and pro-B cells are shown. BACs (shown in green) were directly labeled with dUTP conjugated to Alexa dyes. Nuclei were visualized by DAPI staining (blue). Nuclear lamina are shown in orange. (b) The graph shows the fraction of spatial distances (<500 nm) separating the Ebf1 domain from the nuclear lamina in pre-pro-B and pro-B cells, respectively. 112 and 102 measurements were done in pre-pro-B and pro-B cells, respectively. (c) PC1 values for the Bcl11b locus in pre-pro-B and pro-B cells are indicated. Data obtained from two experiments were added and presented.