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. Author manuscript; available in PMC: 2013 Dec 13.
Published in final edited form as: Brain Res. 2012 Oct 26;1489:66–80. doi: 10.1016/j.brainres.2012.10.035

Figure 5. SMN knockdown in muscle affects the quantal size of pre-synaptic transmitter release.

Figure 5

(A, B) Representative traces and quantification for baseline synaptic transmission at the third instar larval NMJ following muscle-specific perturbation in SMN. Both over-expression and knockdown of SMN result in smaller EJP and mEJP amplitudes. As a result quantal content is not significantly altered in experimental animals. 9–10 animals are tested for each genotype. Vertical scale bar = 10 mV for EJP and 5 mV for mEJP; Horizontal scale bar = 100 ms for EJP and 200 ms for mEJP. Mean resting membrane potentials are: Control = 74mV, UAS-SMN = 66mV, SMN-RNAi-C24 = 63mV, SMN-RNAi-N4 = 62mV. Mean muscle input resistances are: Control = 13.2MΩ, UAS-SMN = 12.4MΩ, SMN-RNAi-C24 = 12.5MΩ, SMN-RNAi-N4 = 11.8MΩ. (C) Staining of third instar synapses with anti-HRP and anti-GluRIII shows indistinguishable staining intensities and pattern of glutamate receptor distribution following SMN knockdown in muscles. Scale bar = 5 μm.