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. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: DNA Repair (Amst). 2012 Oct 17;11(12):942–950. doi: 10.1016/j.dnarep.2012.09.003

Fig. 5.

Fig. 5

UV-induced RNAPII Ser5-phosphorylation and degradation in XP-B/CS fibroblasts. The exponentially growing NHF (A), XPCS1BA, XPCS2BA (B), XP181MA and XP183MA (C) fibroblasts were UV-irradiated at a dose of 20 J/m2 and maintained for indicated repair period in fresh medium. The protein extracts were then made in SDS lysis buffer, and the proteins were quantitated and examined by Western blotting using specific anti-XPB and anti-phospho-RNAPII or anti-β-actin antibodies. (D) Representative Western blot images were quantitated using ImageJ software and arbitrary amount of RNAPII were estimated relative to protein levels found without UV irradiation.