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. Author manuscript; available in PMC: 2013 Nov 16.

Published in final edited form as: Biochem Biophys Res Commun. 2012 Oct 15;428(2):245–251. doi: 10.1016/j.bbrc.2012.10.029

Fig. 1 — Time course for APAP hepatotoxicity in Jα18^−/− mice and WT mice. (A) Serum ALT levels. (B) Representative H & E staining of liver sections from WT and Jα18^−/− mice. (C) HPLC measurement of hepatic GSH levels in WT and Jα18^−/− mice before and after APAP treatment. Data in (A) and (C) are presented as mean ± SEM with n=6–12 mice/group with *p<0.05 vs. naïve WT; ^#p<0.05 vs. corresponding WT as assessed by ANOVA/Newman-Keuls post hoc test. (D–E) Effect of GSH depletion on APAP liver toxicity in Jα18^−/− mice. BSO or PBS was injected in WT and Jα18^−/− mice (see methods) for assessment of serum ALT levels (D) and Histological injury by H & E staining of liver sections (E). Data in D is shown as mean ± SEM with n=7–19 mice/group with *p<0.05 vs. WT/PBS; ^#p<0.05 vs. WT/PBS/APAP; ^op<0.05 vs. Jα18^−/−/PBS/APAP.

Fig. 1 — Time course for APAP hepatotoxicity in Jα18^−/− mice and WT mice. (A) Serum ALT levels. (B) Representative H & E staining of liver sections from WT and Jα18^−/− mice. (C) HPLC measurement of hepatic GSH levels in WT and Jα18^−/− mice before and after APAP treatment. Data in (A) and (C) are presented as mean ± SEM with n=6–12 mice/group with *p<0.05 vs. naïve WT; ^#p<0.05 vs. corresponding WT as assessed by ANOVA/Newman-Keuls post hoc test. (D–E) Effect of GSH depletion on APAP liver toxicity in Jα18^−/− mice. BSO or PBS was injected in WT and Jα18^−/− mice (see methods) for assessment of serum ALT levels (D) and Histological injury by H & E staining of liver sections (E). Data in D is shown as mean ± SEM with n=7–19 mice/group with *p<0.05 vs. WT/PBS; ^#p<0.05 vs. WT/PBS/APAP; ^op<0.05 vs. Jα18^−/−/PBS/APAP.