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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Mol Cancer Res. 2012 Jul 13;10(11):1440–1450. doi: 10.1158/1541-7786.MCR-12-0158

Figure 1. Inducible suppression of GRM1.

Figure 1

(A.) Suppression of GRM1 protein expression in the ecdysone regulated system in C8161 human melanoma cells in the presence of the inducer, 10 μM Ponasterone A (PonA) in two independent clones. No suppression is observed in control C8161 pVgRXR cells in the presence of PonA. Samples were normalized to α-Tubulin. NT- No treatment, Veh - (Vehicle) DMSO. (B.) Quantitation of C8161 pVgRXR siGRM1 clones A10 and A13 western blots from A. using Optiquant software to determine % change in intensity. (C.) Suppression of GRM1 expression using the tetracycline/doxycycline regulated system in two independent clones from 1205 Lu and UACC903 human melanoma cell lines in the presence of 10 ng/ml doxycycline. No suppression is observed in control 1205 Lu TetR and UACC903 TetR in the presence of doxycycline. Equal loading was shown by levels of α-Tubulin. NT-No treatment, Dox- Doxycycline. (D.) Quantitation of western blots of 1205 Lu TetR siGRM1 and UACC903 TetR siGRM1 clones from panel C using Optiquant software to determine % change in intensity.