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. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: Shock. 2012 Dec;38(6):598–606. doi: 10.1097/SHK.0b013e318273621a

Figure 3. Splenocyte cytokine and chemokine secretion after exposure to PRBC, preservative solution, or LPS.

Figure 3

One million spleen cells from naïve mice were treated with either LPS (1 µg/ml), PRBC (30% volume), or CPD and Adsol solution (30% volume). Cells in medium only served as untreated controls. Culture supernatants were collected 24 hours later and assessed for cytokine/chemokine production using multiplex Luminex kit. ****p<0.0001, one-way ANOVA.