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Time course of plasmalemma H+-ATPase activity of 3-week-old corn roots grown at pH 6.0 (○) and 3.5 (•). The H+-ATPase activity of plasmalemma was assayed at 25°C in a solution with 5 mm BTP/Mes (pH 6.5), 5 mm Mg-ATP, 100 mm KCl, 7.5 μm AO, and 0.05% (w/v) Brij 58. A representative example of four replications is presented.
