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. Author manuscript; available in PMC: 2013 Jun 22.
Published in final edited form as: J Membr Biol. 2012 Jun 22;245(5-6):221–230. doi: 10.1007/s00232-012-9443-5

Fig. 2.

Fig. 2

ER-retained Cx40 is monomeric. a, b HeLa cells transfected with either Cx40 (a) or Cx40-HKKSL (b) were fixed and immunostained. Untagged Cx40 was transported to the plasma membrane, where it formed gap junction plaques (arrowhead). By contrast, Cx40-HKKSL was retained in the ER. c HeLa/Cx40-HKKSL cells (squares) or HeLa/Cx43-HKKSL cells (dashed line) were processed and analyzed by sucrose gradient fractionation. Cx40-HKKSL had a similar profile on the gradient as Cx43-HKKSL, indicating that the ER-resident pool was monomeric. Bar 10 µm