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. 2012 Nov 15;61(12):3247–3254. doi: 10.2337/db12-0441

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© 2012 by the American Diabetes Association.

Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered. See http://creativecommons.org/licenses/by-nc-nd/3.0/ for details.

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FIG. 5. — A: Fluorescence microscopy revealed lower protein expression of B2M in MN-siB2M–treated grafts compared with control probe grafts 2 weeks after adoptive transfer (green, insulin; red, B2M; blue, DAPI nuclear stain; magnification bar = 50 μm). B: Fluorescence microscopy showed markedly lower CD8⁺ T-cell infiltration in MN-siB2M–treated grafts compared with control islet grafts 2 weeks after adoptive transfer (green, insulin; red, CD8; blue, DAPI nuclear stain; magnification bar = 40 μm). C: Western blot analysis confirmed notably lower B2M protein expression in MN-siB2M–treated islet grafts compared with control grafts. (A high-quality digital representation of this figure is available in the online issue.)