FIG. 7.

Nrf2 activation pharmacologically impaired hormone-induced adipogenesis and differentiation in MEFs. A: Representative image of Oil Red O staining of MEFs preactivated Nrf2 with sulforaphane (SFN; 10 μmol/L, 12 h) that were induced to differentiate to adipocytes for 6 days (100×, scale bar = 100 μm). B: MEFs were pretreated with the Nrf2 activator of sulforaphane (10 μmol/L, 6 h) and induced to differentiate to adipocytes for 24 h; total protein was extracted, and immunoblot analysis of Nrf2 was performed as indicated. C: Total RNA was extracted from MEFs pretreated with sulforaphane and induced to differentiate for 3 days, and the indicated mRNA level was measured by quantitative real-time PCR. D: Representative image of Oil Red O staining of MEFs induced to differentiate to adipocytes for 3 days. MEFs were then treated with sulforaphane or not (control [CON]) for the remaining days while incubating with differentiated media (100×, scale bar = 100 μm). Cells were fixed at day 6, day 8, and day 10. *P < 0.05, sulforaphane treatment (SFN) compared with CON.