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Copurification of Cx43 and Cx37 suggests that they form heteromeric connexons. Triton X-100 soluble material (containing connexons) from EcR293 cells transfected with HA-tagged Cx37 was affinity purified using an anti-HA resin. Fractions of the initial extract, various column fractions, and the eluate were analyzed by immunoblotting. Even though only Cx37 contained the HA-tag, both Cx37 and Cx43 were detected in the eluate
