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. 2012 Nov 20;135(11):3336–3347. doi: 10.1093/brain/aws250

Figure 3.

Figure 3

S100B and RAGE messenger RNA and protein in mouse ventral midbrain area containing the substantia nigra after MPTP. In mice, real-time polymerase chain reaction and western blot tests indicate that S100B messenger RNA and protein levels are increased directly at Day 0 after MPTP treatment. Within 3 weeks, S100B protein levels returned back to base levels, whereas messenger RNA levels dropped even below base levels (A and C). RAGE protein levels are increased 2 days after MPTP treatment and then drop back to base levels, whereas RAGE messenger RNA levels drop below base levels (B and D). Double immunofluorescence reveals that S100B protein (green) co-localizes with GFAP, an astrocytic marker (red; E–G and an enlarged 3D inset, N). S100B is not detectable in tyrosine hydroxylase neurons (red; H–J) but can be found in single ionized calcium-binding adaptor molecule 1 (Iba1)-positive cells (red; K–M). Data are mean ± SEM for four to six mice per group. *P < 0.05 compared with saline (Newman–Keuls post hoc test).