Figure 4. Characterization of the Ab contribution to successful vaccination.
A. Ab titers against WNV E (Left panel) and NS1 (Right panel) from C57BL/6 mice immunized with 105 IU of RepliVAX WN and challenged with WNV as determined by ELISA on day 45 post-vaccination, and day 6 and 45 post-challenge. Results depict x+/− SEM from n=4–20 mice per group from one of two similar experiments and statistical differences are represented in the figure. B. Neutralizing antibody titers of pooled serum from old and adult animals. Serum was collected45 days post-vaccination and 6 days, 14 days, and 6 months post-challenge and assayed for neutralizing capacity. n=8 mice per group. C. Serum transfer from vaccinated old and adult B6 mice to μMT−/−. μMT−/− mice that received serum from adult vaccinated mice survived WNV challenge (2000 pfu WNV, i.p.) better than those that received serum from old vaccinated mice (p<0.0001). Compiled data from two experiments, n=10–20 mice per group. D. Serum transfer from vaccinated old and adult B6 mice to RAG−/−. RAG−/− mice that received serum from adult vaccinated mice survived WNV infection (1000 pfu WNV, s.c.) longer than those that received serum from old vaccinated mice (p=0.0289) but had no difference in overall survival percentages. n=8 mice per group. E. Serum transfer from vaccinated and challenged old and adult B6 mice to μMT−/− from day 6 post-challenge. μMT−/− mice that received serum from adult vaccinated mice survived WNV infection (2000 pfu WNV, i.p.) better than those that received serum from old vaccinated mice (p=0.0002). n=8 mice per group. F. Serum transfer from vaccinated and challenged old and adult B6 mice to μMT−/− from day 14 post-challenge. μMT−/− mice that received serum from adult vaccinated mice survived WNV infection (2000 pfu WNV, i.p.) equally as well as those that received serum from old vaccinated mice (p=ns). n=8 mice per group.)