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. 1998 May;117(1):43–53. doi: 10.1104/pp.117.1.43

Figure 3.

Figure 3

Presence of EP3 chitinases in media of nonembryogenic, embryogenic, and density-fractionated embryogenic cultures. A, Detection of EP3 isoenzymes in the medium of several different suspension cultures. Lanes 1 and 2, Nonembryogenic suspension cultures; and lanes 3 to 6, embryogenic suspension cultures. B, Detection of EP3 isoenzymes in the medium of a Percoll-fractionated suspension culture: lane 1, cells < 50 μm; lane 2, 0 to 10% Percoll; lane 3, 10 to 20% Percoll; lane 4, 20 to 30% Percoll; lane 5, 30 to 40% Percoll; and lane 6, cells > 125 μm. The proteins were separated by nondenaturing PAGE and detected by immunoblotting.